Yes. Payer/ Deductor needs to use separate challans to deposit tax deducted (TDS) for different types of
deductees,i.e. Corporate or Non-Corporate, and indicate the type of deductees in the relevant column in the challan no. 281. 

For example, Select corporate deductees, indicate “COMPANY DEDUCTEES - 0020” if you are depositing tax deducted on payment made to a company or LLP or Firm. Select “NONCOMPANY DEDUCTEES - 0021”  and for non-corporate deductees like individuals.